A Melting Curve Analysis–Based PCR Assay for One-Step Genotyping of β-Thalassemia Mutations
نویسندگان
چکیده
منابع مشابه
Improved high sensitivity screen for Huntington disease using a one-step triplet-primed PCR and melting curve assay
Molecular diagnosis of Huntington disease (HD) is currently performed by fluorescent repeat-flanking or triplet-primed PCR (TP-PCR) with capillary electrophoresis (CE). However, CE requires multiple post-PCR steps and may result in high cost in high-throughput settings. We previously described a cost-effective single-step molecular screening strategy employing the use of melting curve analysis ...
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ژورنال
عنوان ژورنال: The Journal of Molecular Diagnostics
سال: 2011
ISSN: 1525-1578
DOI: 10.1016/j.jmoldx.2011.03.005